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Our protocol establishes a closer match of mouse 2-cell (2C) embryos than any other 2C-like cells. In the case of humans, the gametes sperm and egg, fuse to form a zygote. Our approach of remodeling the pericentromeric heterochromatin and re-establishing the totipotency-specific broad H3K4me3 domains promotes the pluri-to-totipotency transition. Totipotent stem cells are embryonic stem cells characterized by their ability to generate all portions of a product of conception including the embryo and extraembryonic tissues such as placental tissues and fetal membranes. Stable totipotent cell cultures and deciphering the principles of totipotency regulation would be invaluable to understand cell plasticity and lineage segregation in early development. Potency is also described as the gene activation potential within a cell, which like a continuum, begins with totipotency to designate a cell with the most differentiation potential, pluripotency, multipotency, oligopotency, and finally unipotency. The more cell types a cell can differentiate into, the greater its potency. ![]() ![]() Totipotent cells have more robust developmental potency than any other cell types, giving rise to both embryonic and extraembryonic tissues. Cell potency is a cell's ability to differentiate into other cell types. ![]() ![]() They are not ideal for general purpose emulation because they’re built around speedhacks, have relatively lower accuracy and are prone to bugs. Developed by Martin Korth, the emulators are available for various classic consoles, including the original PlayStation, GBA, NDS, Atari 2600, Commodore 64, SNES and more. 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